LAYNE NORTON MUSCLE PROTEIN SYNTHESIS PDF

However, the real question is does eating more provide metabolic benefits? That has yet to be seen. The problem with both of these is they do not account for recycling of the amino acids within various tissues, nor do they take into account that tissues like the gut turnover very rapidly and so much of the whole body flux of amino acids is from the gut. Skeletal muscle turns over comparatively slowly and thus has much smaller effects on whole body amino acid fluxes. In order to get good information you have to look at fractional rates of synthesis and degradation within the skeletal muscle which 1 is much more difficult 2 more expensive 3 requires more skill and 4 requires a biopsy.

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On d 15, rats were food-deprived for 12 h and then fed their normal treatment 4 g breakfast meal. The food-deprived controls received no breakfast meal. The meals provided 0, 46, 54, 60, and 74 mg of Leu for the food-deprived controls, wheat, soy, egg, and whey groups, respectively.

Rats were killed 90 min after consumption of the meal and blood and tissue samples collected. MPS was measured at 0 and min time-points as described below. Experiment 2 examined supplementing the wheat gluten meal with Leu to determine if matching Leu contents of the wheat and whey meals would yield similar peak rates of postprandial MPS.

Based on findings in Experiment 1 and our previous research [ 7 ] demonstrating that the MPS meal response was the same after a single meal or after 14 day feeding, Experiment 2 was performed as a single meal study.

All rats were adapted to meal feeding as described in Experiment 1. After 6 day of adaptation to meal feeding, rats were assigned to treatment groups based on body weight. The whey protein and wheat gluten diets were supplemented with glycine Sigma-Aldrich, St. Post-meal responses in plasma Leu, were measured at 30, 90, and min after completion of the meal to determine if supplementing free Leu altered the pattern of Leu appearance in the blood.

Rats were euthanized and blood and tissues harvested as in Experiment 1. MPS was measured at 0 and min time-points. Determination of Muscle Protein Synthesis Protein synthesis was measured in gastrocnemius muscles using the flooding dose method [ 12 ]. After 10 min rats were killed by decapitation and hind limbs quickly removed and immersed in an ice-water mixture.

The resulting supernatant and protein pellet were prepared for gas chromatography mass spectroscopy GC-MS analyzes as described previously [ 13 , 14 ]. Muscle supernatants were used for determination of intracellular free phenylalanine enrichment. Fractional rates of protein synthesis MPS were determined from the rate of incorporation of L-2 H5-phenylalanine into total mixed muscle protein as described previously [ 16 ].

The time from injection of the metabolic tracer until tissue cooling was recorded as the actual time for L-2 H5-phenylalanine incorporation. Charles, MO. When variances were not homogeneous, means were compared using a Games-Howell test. Results Experiment 1 This experiment compared isonitrogenous, isoenergetic meals containing wheat gluten, soy protein isolate, egg white protein, or whey protein isolate as the protein source on the potential to initiate translation and protein synthesis in skeletal muscle.

Similar post-prandial patterns occurred for each of the BCAA with whey producing the highest concentrations at 90 min after the meal i. Table 4 Experiment1: Selected plasma essential amino acid1and glucose2concentrations 90 min after feeding meals containing wheat, soy, egg, or whey proteins

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Leucine: The Anabolic Trigger!

On d 15, rats were food-deprived for 12 h and then fed their normal treatment 4 g breakfast meal. The food-deprived controls received no breakfast meal. The meals provided 0, 46, 54, 60, and 74 mg of Leu for the food-deprived controls, wheat, soy, egg, and whey groups, respectively. Rats were killed 90 min after consumption of the meal and blood and tissue samples collected. MPS was measured at 0 and min time-points as described below. Experiment 2 examined supplementing the wheat gluten meal with Leu to determine if matching Leu contents of the wheat and whey meals would yield similar peak rates of postprandial MPS. Based on findings in Experiment 1 and our previous research [ 7 ] demonstrating that the MPS meal response was the same after a single meal or after 14 day feeding, Experiment 2 was performed as a single meal study.

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About Layne

Tojajin Do you calculate the amount of protein coming from your carb sources along with your protein as your total protein count for the day. I think whey post workout is fine, but really i just use it whenever i need to get some extra quick protein in. What Does Catabolic Mean? In the small intestine the layme protein is then cleaved into individual amino acids, di, and tri-peptides. Leucine: The Anabolic Trigger! The contracting company is mTOR, the skyscraper is the protein you are trying to synthesize, the machines bulldozers, cranes, etc you use to make the building are the protein synthesis pathway components, and leucine is the cash needed to make the project work. It actually covers my questions.

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