Red cells indicate the cells expressing the exogenous genes. The original set of reprogramming factors also dubbed Yamanaka factors are the transcription factors Oct4 Pou5f1 , Sox2 , cMyc , and Klf4. While this combination is most conventional in producing iPSCs, each of the factors can be functionally replaced by related transcription factors, miRNAs , small molecules, or even non-related genes such as lineage specifiers. However, considerable advances have been made in improving the efficiency and the time it takes to obtain iPSCs. Upon introduction of reprogramming factors, cells begin to form colonies that resemble pluripotent stem cells, which can be isolated based on their morphology, conditions that select for their growth, or through expression of surface markers or reporter genes. They chose twenty-four genes previously identified as important in ESCs and used retroviruses to deliver these genes to mouse fibroblasts.
|Published (Last):||11 December 2009|
|PDF File Size:||15.59 Mb|
|ePub File Size:||6.82 Mb|
|Price:||Free* [*Free Regsitration Required]|
Vucage Germ-line transmission of genes introduced into cultured pluripotential cells by retroviral vector. Cloned pigs produced by nuclear transfer from adult somatic cells. Reprogramming of human somatic cells to pluripotency with defined factors. Human embryonic germ cell derivatives express a broad range of developmentally distinct markers and proliferate extensively in vitro. Junying Yu, Maxim A. Multi lineage differentiation from human embryonic stem cell lines.
Site-directed mutagenesis by gene targeting in mouse embryo-derived stem cells. Primate parthenotes yield stem cells. First cloned mule races to finish line. Faithful expression of imprinted genes in cloned mice. Production of goats by somatic cell nuclear transfer. PBMNCs Centrifugar a xg durante 10 pluripotenciiales a temperatura ambiente, aspirar el sobrenadante y se descartan.
Nuclear transplantation in eggs and oocytes. Feeding tadpoles cloned from Rana pluripoteniales nuclei. Characterization and culture of human embryonic stem cells. Targeted disruption of the alpha1,3-galactosyltransferase gene in cloned pigs. Derivation of oocytes from mouse embryonic stem cells. Nuclear transfer into mouse zygotes.
Embryonic and extraembryonic stem cell lines derived from single mouse blastomeres. Generation of germline-competent induced pluripotent stem cells. In-utero overgrowth in ruminants following embryo culture: Embryonic stem cells differentiate into oligodendrocytes and myelinate in culture and after spinal cord transplantation.
Cloning of male mice from adult tail-tip cells. Please sign inducieas or create an account. Epub Jun Stem cells may shore up transplanted hearts. Isolation of a pluripotent cell line from early mouse embryos cultured in medium conditioned by teratocarcinoma stem cells.
A comparison of the germline potential of differently aged ES cell lines and their transfected descendants. El uso de capas leucocitarias en lugar de gradiente de clulas separadas PBMNCs todosujos nos permite reducir costos, tiempo de trabajo y los pasos manuales para los operadores durante el procesamiento de la muestra. Correction of a genetic defect by nuclear transplantation and combined cell and gene therapy. Nuclear cloning and epigenetic reprogramming of the genome. Gurdon JB, Colman A.
Cloning sheep from cultured embryonic cells. Evaluation of gestational deficiencies in cloned sheep fetuses and placentae. Please check your Internet connection and reload this page. Skip to content Developmental Biology. Pluripotency of mesenchymal stem cells derived from adult marrow. Nuclear transfer technology in mammalian cloning. Most Related.
Célula madre pluripotente inducida
La edad del donante influye en la calidad de las células madre pluripotentes inducidas
Diferencia entre células madre pluripotentes y multipotentes