JCM 5046 PDF

This article has been cited by other articles in PMC. However, Southern blot analysis revealed that the bla BKC-1 gene was located on the same kb plasmid in both isolates. Frequency of BKCproducing Ucm species isolates. These data might suggest that, although genetically related, the isolates were probably subjected to distinct selective pressures, which could have resulted in different OMP modifications. It is important to note that such new studies must search for bla BKC-1 using molecular techniques, since phenotypic tests may not accurately detect BKCproducing isolates 8.

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Gorisar However, Southern blot analysis revealed that the bla BKC-1 gene was located on the same kb plasmid in both isolates.

Comparison of phenotypic tests for detecting BKCproducing Enterobacteriaceae isolates. In conclusion, prudent use of antimicrobials and strict adherence to infection control measures seem to be the only effective tools for preventing the acquisition and spread of new carbapenemase-encoding genes. Please review our policy. Jck, we performed transformation by electroporation into Escherichia coli Top Abstract BKC-1 is a new class A serine carbapenemase that was recently identified in Klebsiella pneumoniae clinical isolates.

Only 2 BKCproducing K. We verified that, in the absence of selective pressure, the plasmid harboring the bla BKC-1 gene, p, was unstable. These carbapenem resistance genes are frequently harbored on mobile genetic elements that are highly transmissible, contributing to the increasing frequency of carbapenem-resistant Enterobacteriaceae 1. National Center for Biotechnology InformationU.

Clinical and Laboratory Standards Institute. Open in a separate window. Prepublished online May During the experiments, it was observed that bla BKC-1 was easily lost in the absence of selective pressure. Since BKC-1 is a weak carbapenemase and other mechanisms could be associated with carbapenemase production, we selected Klebsiella strains independent of the resistance phenotype. Jcmmb Jorge L. The Kpn and Ucm strains showed resistance to all beta-lactams and fluoroquinolones.

Among the Klebsiella strains, It is important to note that such new studies must search for bla BKC-1 using molecular techniques, since phenotypic tests may not accurately detect BKCproducing isolates 8. The mgrB gene as a key target for acquired resistance to colistin in Klebsiella pneumoniae. Multilocus sequence typing of Klebsiella pneumoniae nosocomial isolates.

Antimicrobial susceptibility testing was performed by the broth microdilution method, according to CLSI guidelines BKC-1 hcm a new class A serine carbapenemase that was recently identified in Klebsiella pneumoniae clinical isolates. A Map of Brazil, showing the geographic distribution of Klebsiella isolates that were investigated for the bla BKC-1 gene. Support Center Support Center. This article has been cited by other articles in PMC. The other authors have no conflicts to declare.

Transformant cells were selected on LB agar plates supplemented with 0. The Klebsiella strains were isolated from different clinical specimens obtained between and Fig.

The Kpn and KpnJ strains showed different plasmid profiles. Carbapenemase-producing Klebsiella pneumoniae, a key pathogen set for global nosocomial dominance.

Jfm addition, bla BKC-1 was harbored by a small unstable plasmid that could be easily lost in the absence of selective pressure. To determine whether this plasmid was identical to p, the first plasmid identified as carrying bla BKC-1we performed whole-plasmid sequencing jc, the plasmid recovered from KpnJ.

J Clin Microbiol Only 2 of Klebsiella isolates 0. These data might suggest that, although genetically related, the isolates mcm probably subjected to distinct selective pressures, which could have resulted in different OMP modifications.

The two BKCproducing isolates belonged to clonal complex and possessed identical pulsed-field gel electrophoresis patterns. The bla BKC-1 gene was inserted into a kb plasmid that was identical to the previously reported plasmid, p Related Posts.

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JCM 5046 PDF

Initially, we performed transformation by electroporation into Escherichia coli Top Since BKC-1 is a weak carbapenemase other mechanisms could be associated with carbapenemase production, we selected Klebsiella strains independent of the resistance phenotype. Published online Jul It is important to note that such new studies must jc for bla BKC-1 using molecular techniques, since phenotypic tests may not accurately detect BKCproducing isolates 8. Please review our privacy policy. Only 2 BKCproducing K.

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Martinsa Adriana G. BKC-1 Brazilian Klebsiella carbapenemase is a class A serine carbapenemase that was recently identified in Klebsiella pneumoniae isolates from Brazil that belonged to sequence type ST clonal complex [CC] 2. Multilocus sequence typing of Klebsiella pneumoniae nosocomial isolates. Author information Article notes Copyright and License information Disclaimer. The two BKCproducing isolates belonged to clonal complex and possessed identical pulsed-field gel electrophoresis patterns. These carbapenem resistance genes are frequently harbored on mobile genetic elements that are highly transmissible, contributing to the increasing frequency of carbapenem-resistant Enterobacteriaceae 1. During the experiments, it was observed that bla BKC-1 was easily lost in the absence of selective pressure.

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Gorisar However, Southern blot analysis revealed that the bla BKC-1 gene was located on the same kb plasmid in both isolates. Comparison of phenotypic tests for detecting BKCproducing Enterobacteriaceae isolates. In conclusion, prudent use of antimicrobials and strict adherence to infection control measures seem to be the only effective tools for preventing the acquisition and spread of new carbapenemase-encoding genes. Please review our policy.

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Bazahn Clinical and Laboratory Standards Institute. Performance standards for antimicrobial susceptibility testing; 25th informational supplement. Open in a separate window. Comparison of phenotypic tests for detecting BKCproducing Enterobacteriaceae isolates. It is important to note that such new studies must search for bla BKC-1 using molecular techniques, since phenotypic tests may not accurately detect BKCproducing isolates 8.

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